Protein_Domain

Part:BBa_K1408000:Experience

Designed by: Stephen A Rettie   Group: iGEM14_UW   (2014-10-09)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1408000

For The University of Washington's 2014 iGEM project we used a fusion protein made up of Gal4 fused to a protein of interest (Bindi, BbpD04, or BbpD04.3) with that then fused to VP16. Gal4-VP16 binds to a promoter on PyE1 yeast which causes the up-regulation of the expression of GFP. We had five possible positions of inserting a degron into this system and characterized each via flow cytometry as seen in Fig1.

Fig1

User Reviews

UNIQ9594a3f4d15b04e0-partinfo-00000000-QINU UNIQ9594a3f4d15b04e0-partinfo-00000001-QINU